Bifidobacterial Diversity in Human Feces Detected by Genus-Specific PCR and Denaturing Gradient Gel Electrophoresis

Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR produ...

Denaturing Gradient Gel Electrophoresis Detected by Genus-Specific PCR and Bifidobacterial Diversity in Human Feces

Study of Bacterial Diversity in Poultry Gut Using Denaturing Gradient Gel Electrophoresis

Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...

Diversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis

PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...

Detection of Lactobacillus, Pediococcus, Leuconostoc, and Weissella species in human feces by using group-specific PCR primers and denaturing gradient gel electrophoresis.

Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that ha...