<b>Identification of X- and Y-chromosome-bearing human sperm separated by free-flow electrophoresis using Y-chromosome-specific polymerase chain </b><b>reaction </b>
نویسندگان
چکیده
منابع مشابه
Flow cytometric determination of the proportions of X- and Y-chromosome-bearing sperm in samples of purportedly separated bull sperm.
A rapid assay for determining the proportions of X- and Y-chromosome-bearing sperm in semen samples would benefit research aimed at sex ratio control through sperm separation. It also would be of value for quality control should a separation technique be developed. Flow cytometric methods capable of measuring sperm DNA content precisely enough to resolve and quantify the X and Y populations in ...
متن کاملComparative motility of X and Y chromosome-bearing bovine sperm separated on the basis of DNA content by flow sorting.
A combination of flow cytometric sperm sorting of X and Y chromosome-bearing sperm (X and Y sperm) and computer-assisted sperm analysis (CASA) for measuring sperm motility allows assessment of motion parameters in the two populations. Bull sperm were separated into X and Y populations by flow cytometry following staining with the DNA-binding dye Hoechst 33,342. The motion parameters differed de...
متن کاملI-49: Human Y Chromosome ProteomeProject
The success of the Human Genome Project (HGP) has provided a blueprint for the approximately 20,000 gene-encoded proteins potentially active in all of the hundreds of cell types that make up the human body. Yet we still have limited knowledge about a majority of the gene-encoded proteins which are the “building blocks of life” and “cellular machinery”. It is estimated that for nearly half of th...
متن کاملI-3: Human Y Chromosome Proteome Project 2012 Update
The Human Genome Project has generated a blueprint for the approximately 20,300 gene-encoded proteins potentially active in any of 230 cell types that make up the human body (human proteome). However, based on the UniProtKB/Swiss-Prot database content, about 6000 of at the protein level; for many others, there is very little information related to protein function, abundance, subcellular locali...
متن کاملY Chromosome-Specific STRs
The Y chromosome is one of the smallest human chromosomes with an average size of 60 million base pairs (Mb). Between X and Y chromosomes, exchange is limited to small pseudoautosomal regions (PAR) of the X-Y pair (see Figure 1). During male meiosis, recombination only takes place at the most distal short arm (PAR 1) and at the tip of the Y chromosome long arm (PAR 2). For most of its length (t...
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ژورنال
عنوان ژورنال: Biomedical Research
سال: 1992
ISSN: 0388-6107,1880-313X
DOI: 10.2220/biomedres.13.221