Beta glucosidase from Bacillus polymyxa is activated by glucose-6-phosphate
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چکیده
منابع مشابه
Crystal structure of beta-glucosidase A from Bacillus polymyxa: insights into the catalytic activity in family 1 glycosyl hydrolases.
Family 1 glycosyl hydrolases are a very relevant group of enzymes because of the diversity of biological roles in which they are involved, and their generalized occurrence in all sorts of living organisms. The biological plasticity of these enzymes is a consequence of the variety of beta-glycosidic substrates that they can hydrolyze: disaccharides such as cellobiose and lactose, phosphorylated ...
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Glucose 6-phosphate dehydrogenase (G6PD) from Streptomyces aureofaciens was purified and denatured in 6 M urea. Denaturation led to complete dissociation of the enzyme into its inactive monomers, 98% loss of the enzyme activity, about 30% decrease in the protein fluorescence and a 10 nm red shift in the emission maximum. Dilution of urea-denatured enzyme resulted in regaining of the enzyme acti...
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Human erythrocyte glucose 6-phosphate dehydrogenase (o-glucose 6-phosphate :nicotinamide adenine dinucleotide phosphate oxidoreductase, EC 1.1.1.49) was purified by column chromatography with diethylaminoethyl cellulose, calcium phosphate gel, carboxymethyl cellulose, diethylaminoethyl Sephadex, and carboxymethyl Sephadex. A homogeneous preparation was obtained in over-all yield of about 50%. T...
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Glucose 6-phosphate dehydrogenase from Zeuconostoc mesenteroides, previously isolated in crystalline form (Olive, C., and Levy, H. R., Biochemistry, 6, 730 (1967)), is shown to be essentially homogeneous by disc gel electrophoresis and sedimentation velocity analysis. The weight average molecular weight is 103,700, determined by both high speed and low speed sedimentation equilibrium techniques...
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Glucose-6-phosphate dehydrogenase (G6PD) from Leuconostoc mesenteroides is one of a small number of dehydrogenases that can utilize either NAD or NADP. The physiological roles of these two coenzymes are quite different and the enzymes that utilize them are generally specific for one or the other coenzyme. An essential function of G6PDs in animals, for example, is to generate NADPH for reductive...
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ژورنال
عنوان ژورنال: Archives of Biochemistry and Biophysics
سال: 2015
ISSN: 0003-9861
DOI: 10.1016/j.abb.2015.06.012