Beta-Elemene Suppresses Metastasis of Human Esophageal Cancer Cell by Downregulating Heat Shock Protein 70/Mothers against Decapentaplegic Homolog 2 Pathway

In this study, we tried to illuminate the role and underlying mechanism of beta-elemene in esophageal squamous cell carcinoma especially metastasis. 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide assay was performed determine cytotoxicity its proliferation. Wound healing transwell assays were used elucidate roles betaelemene on migration invasion abilities cells. Quantitative polymerase chain reaction western blot carried out assess messenger RNA protein expression under treatment. Immunofluorescence staining detect heat shock 70 regulated by beta-elemene. The ectopic plasmid perform rescue experiments. found that significantly inhibited survival proliferation suppressed line. Mechanistically, observed downregulated expressions reversed epithelial-mesenchymal transition. Furthermore, studies also indicated phosphorylation extracellular kinase, mothers against decapentaplegic homolog 2 3 expression. addition, confirmed not only promoted migration, epithelialmesenchymal transition line cells, but TE-1 Taken together, all results elucidated suppresses metastasis human modulating activation kinase transforming growth factor beta signaling pathways at least partially.