Ballistic Disruption of Penicillium chrysogenum Cells
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چکیده
منابع مشابه
Ballistic disruption of Penicillium chrysogenum cells.
The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in...
متن کاملPenicillin acyltransferase in Penicillium chrysogenum.
Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...
متن کاملThe lactic dehydrogenase of Penicillium chrysogenum.
A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...
متن کاملA study of lipides of Penicillium chrysogenum.
Relatively few studies have been made on the lipide metabolism of Penicillium chrysogenum, although large quantities of these compounds are used as antifoam agents in the production of penicillin. Goldschmidt and Koffler (1) demonstrated that unsaturated long chain fatty acids present in the antifoam were responsible for stimulation of penicillin yields, since many physiologically inert compoun...
متن کاملGene targeting in Penicillium chrysogenum: disruption of the lys2 gene leads to penicillin overproduction.
Two strategies have been used for targeted integration at the lys2 locus of Penicillium chrysogenum. In the first strategy the disruption of lys2 was obtained by a single crossing over between the endogenous lys2 and a fragment of the same gene located in an integrative plasmid. lys2-disrupted mutants were obtained with 1.6% efficiency when the lys2 homologous region was 4.9 kb, but no homologo...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1962
ISSN: 0003-6919
DOI: 10.1128/am.10.6.577-579.1962