Bactobilin: blue bile pigment isolated from Clostridium tetanomorphum.

Threonine Deaminase of Clostridium tetanomorphum

Dissociation of the threonine deaminase of Ctostridium tetanomorfihum occurs upon exposure to heat, to alkaline pH, or to denaturing agents, or when preparations are diluted or allowed to age at -20°. Dissociation in the presence of urea, detergents, or an alkaline pH may be prevented by addition of adenosine diphosphate, threonine, phosphate buffer at pH 7.0, or monovalent or divalent cations....

Threonine Deaminase of Clostridium tetanomorphum

Threonine deaminase (Lthreonine hydrolyase (deaminating), EC 4.2.1.16) has been purified approximately 700-fold from extracts of Clostridium tetanomorphum. Both threonine and serine can serve as substrates, but threonine is deaminated 5 to 10 times more rapidly than serine. Pyridoxal phosphate, a reducing agent, and alkaline pH are required for the deamination of either amino acid. A plot of th...

Clostridium perfringens and other anaerobes isolated from bile.

Clostridium perfringens was isolated from bile in 13 cases of 150 patients examined. The serotypes of C perfringens strains isolated from bile and faeces were investigated using antisera to Hobbs' type 1-17. Two or more serological types were often found in a single specimen, but in the same patient the serotypes of C perfringens strains isolated from the bile were identical with those from the...

Deferred metabolism of glucose by Clostridium tetanomorphum.

Outstanding bile pigment problems.

In this review of the chemistry, biochemistry and chemical pathology of the bile pigments I am well aware that I shall ask many more questions than I am able to answer. It seems appropriate that the subject should be considered under the five headings: -Chemistry; Formation from Haem Proteins; Transport in the Blood; Conjugation and Transport to the Bile; and Changes in the Gut. I shall conclud...