Bacteriological Profile and Detection of β-Lactamase Producing Bacteria Isolated from Blood Samples of Neonates

The clinical impact of β-lactamase has become a public health problem around the world in terms increased morbidity and mortality, especially child population. This study was aimed at determining bacteriological profile detection producing bacteria isolated from blood samples neonates. For this study, total 1335 neonates admitted NICU, SCBU, sepsis-suspected visiting Paropakar Maternity Women’s Hospital, Thapathali, Kathmandu, Nepal were collected processed. Blood culture performed identification done by following standard microbiological methods. Antibiotic susceptibility testing using Kirby Bauer Disk Diffusion method confirmation ESBL, MBL, KPC-producing Combined Test. prevalence rate neonatal sepsis found to be 17%. K. pneumoniae 116 (50.2%) predominant Gram-negative followed oxytoca 31 (13.4%) whereas S. aureus 39 (16.9%) Gram-positive causing sepsis. Among 182 bacterial isolates, 69 (37.9%), 22 (12.1%), 14(31.1%) KPC producers respectively. (54.8%), Enterobacter spp. (25%) Citrobacter (14.3%) co-production also among 5 bacteria. Colistin, Meropenem, Imipenem seem choice drug against bacteria, Vancomycin Cefoxitin Therefore, lessen burden antibiotic resistance, it is essential conduct regular antimicrobial surveillance, periodic reviews hospital settings, early beta-lactamase-producing Int. J. Appl. Sci. Biotechnol. Vol 11(2): 66-77.