Bacterial Surface Display of Levansucrase of Zymomonas mobilis Using Bacillus Subtilis Spore Display System
نویسندگان
چکیده
منابع مشابه
A system of vectors for Bacillus subtilis spore surface display
BACKGROUND Bacterial spores have been utilized as platforms for protein display. The best studied display systems are based on Bacillus subtilis spores in which several coat proteins have successfully been used as anchors for heterologous protein. Increasing knowledge about spore coat structure enables selection of new anchor proteins such as CotZ and CgeA. Here we describe a system of vectors ...
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Endospores of Bacillus subtilis have been used extensively as a platform for recombinant protein display for nearly two decades. Main use of the spore surface display system is generation of oral vaccines against many human and animal pathogens. Formulation of oral vaccine based on spores is an attractive approach to alternative vaccination due to the timesaving and relative easiness of product...
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Display of proteins like feed enzymes at the surface of bacterial spore systems has a great potential use for animal feed. Feed enzymes increase the digestibility of nutrients, leading to greater efficiency in the manufacturing of animal products and minimizing the environmental 5 impact of increased animal production. To deliver their full potential in the gut, feed enzymes must survive the ha...
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We developed a novel surface display system based on the use of bacterial spores. A protein of the Bacillus subtilis spore coat, CotB, was found to be located on the spore surface and used as fusion partner to express the 459-amino-acid C-terminal fragment of the tetanus toxin (TTFC). Western, dot blot and fluorescent-activated cell sorting analyses were used to monitor TTFC surface expression ...
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The refolding of levansucrase denatured by urea was studied as a possible model for the second step of the secretion pathway of this protein. The folding-unfolding transition was monitored by measuring intrinsic fluorescence and resistance to proteolysis. Both methods provided the same estimation for the unfolding free energy of levansucrase, AGD, which was 30.1 + 1.7 kJ mol-1 (7.2 + 0.4 kcal m...
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ژورنال
عنوان ژورنال: KSBB Journal
سال: 2011
ISSN: 1225-7117
DOI: 10.7841/ksbbj.2011.26.3.243