Bacterial plasmolysis as a physical indicator of viability
نویسندگان
چکیده
منابع مشابه
Bacterial plasmolysis as a physical indicator of viability.
Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M NaCl solution, were used as a rapid, growth-independent method for quantifying t...
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Representatives of 15 nematode genera were viewed with 450-490-nm epi-illumination and found to autofluoresce. The autofluorescence was limited to 1-5-mum-d globules in the intestinal cells of live nematodes. When adult Pratylenchus penetrans or Caenorhabditis elegans were killed with formaldehyde, freezing, or heat, autofluorescence dispersed throughout the body. Mixed stages of P. penetrans w...
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The use of propidium iodide, whose uptake indicates cell death or damage, was investigated to assess the viability of heat-inactivated and chemically inactivated Giardia muris cysts. This was done by comparing propidium iodide staining with excystation. We first determined that propidium iodide could be used with an immunofluorescence detection procedure by showing that the percentages of Giard...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1996
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.62.11.3939-3947.1996