Bacterial Outer Membrane Vesicles as Nano?Scale Bioreactors: A Fatty Acid Conversion Case Study
نویسندگان
چکیده
Bacterial outer membrane vesicles (OMVs) are small unilamellar proteoliposomes, involved in various functions including cell-to-cell signalling and protein excretion. We have engineered the OMVs of Escherichia coli to nano-scaled bioreactors for biotransformation fatty acids by targeting a acid double bond hydratase Stentrophomonas maltophilia (SmOhyA) and/or photoactivated decarboxylase from Chlorella variabilis NC64 A (CvFAP) into OMVs. Engineered containing both SmOhyA CvFAP were able catalyse transformation oleic ((Z)-octadec-9-enoic acid) 9-hydroxyheptadecane via (R)-10-hydroxyoctadecanoic acid. The specific rates reached 8.0×10?12 ?mol/min per OMV.
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ژورنال
عنوان ژورنال: Chemcatchem
سال: 2021
ISSN: ['1867-3899', '1867-3880']
DOI: https://doi.org/10.1002/cctc.202100778