AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells
نویسندگان
چکیده
منابع مشابه
AZT on telomerase activity and cell proliferation in HS 839.T melanoma cells.
PURPOSE To evaluate telomerase activity and proliferation of HS839.T melanoma cells, subjected to the action of AZT. METHODS Cells were grown in triplicate, AZT at different concentrations: 50, 100 and 200 μM, was added and left for 24 and 48 hours, and its effects were compared with the control group. Telomerase activity was detected by PCR and cell proliferation was evaluated by MTT. RESU...
متن کاملStudy of telomerase activity, proliferation and differentiation characteristics in umbilical cord blood mesenchymal stem cells
In recent years, considerable advances have been made in the field of regenerative medicine. Unlikeembryonic stem cells, which pose the problems of ethical concerns and cause severe immunological reactions as well as neoplasma formation after transplantation, umbilical cord blood is a primitive source ofmesenchymal stem cells that covers the benefits of both embryonic and adult stem cells. It h...
متن کاملAZT-mediated inhibition of telomerase activity suppresses pancreatic cancer cell proliferation and enhances their radiosensitivity
Objective: To investigate the effects of AZT-mediated inhibition of telomerase activity on the proliferation, apoptosis and radiosensitivity of panc1 cells, and the growth of pancreatic tumor in vivo. Methods: panc1 cells were divided into four groups: A, control group; B, 0.4 mmol/L AZT; C, 0.6 mmol/L AZT; and D, 0.8 mmol/L AZT group. Cell proliferation was assessed by MTT assay. Cell apoptosi...
متن کاملEffect of 3`-Azido-3`-Deoxythymidine (AZT) on Telomerase Activity and Proliferation of HO-8910 Cell Line of Ovarian Cancer
OBJECTIVE To study the effect of 3`-azido-3`-deoxythymidine (AZT) on telomerase activity and the proliferation of ovarian cancer cells in vitro. METHODS Telomerase activity was detected by enzyme linked immunosorbent assay (ELISA) in treated and untreated HO-8910 cells by AZT. The detection of cell viability was performed with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyl tetrazolium bromide (MTT...
متن کاملThe Influence of Iron Loading and Iron Chelation on the Proliferation and Telomerase Activity of Human Peripheral Blood Mononuclear Cells
Background: Iron is an essential trace element in cell proliferation. Several investigations demonstrate that iron deprivation inhibits cell proliferation. However, the impact of iron on telomerase activity of activated lymphocytes remains unexplained to date. Objective: In this study, the effect of iron on the proliferation and telomerase activity of lymphocytes stimulated by phytohemagglutini...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Cirurgica Brasileira
سال: 2012
ISSN: 0102-8650
DOI: 10.1590/s0102-86502012001200005