Autoregulation of fixK 2 gene expression in Bradyrhizobium japonicum
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چکیده
منابع مشابه
Gene Probe Designing for Evaluation of the Diversity of Bradyrhizobium japonicum Isolates
Many researchers consider the use of different probes for hybridization assays as suitable for studying the genetic diversity of nitrogen fixing bacteria. In this study for asessing genetic diversity among Bradyrhizobium japonicum isolates, two different probes (sucA and topA) chosen from the chromosomal genome of Bradyrhizobium strain USDA 110 were designed, evaluated by DNAMAN software and im...
متن کاملIdentification of a functional fur gene in Bradyrhizobium japonicum.
The recent identification of the iron response regulator (Irr) in Bradyrhizobium japonicum raised the question of whether the global regulator Fur is present in that organism. A fur gene homolog was isolated by the functional complementation of an Escherichia coli fur mutant. The B. japonicum Fur bound to a Fur box DNA element in vitro, and a fur mutant grown in iron-replete medium was derepres...
متن کاملConservation of symbiotic nitrogen fixation gene sequences in Rhizobium japonicum and Bradyrhizobium japonicum.
Southern hybridization with nif (nitrogen fixation) and nod (nodulation) DNA probes from Rhizobium meliloti against intact plasmid DNA of Rhizobium japonicum and Bradyrhizobium japonicum strains indicated that both nif and nod sequences are on plasmid DNA in most R. japonicum strains. An exception is found with R. japonicum strain USDA194 and all B. japonicum strains where nif and nod sequences...
متن کاملPositive control of ferric siderophore receptor gene expression by the Irr protein in Bradyrhizobium japonicum.
Ferric siderophore receptors are components of high-affinity iron-chelate transport systems in gram-negative bacteria. The genes encoding these receptors are generally regulated by repression. Here, we show that the ferrichrome receptor gene bll4920 and four additional putative ferric siderophore receptor genes in Bradyrhizobium japonicum are positively controlled by the regulatory protein Irr,...
متن کاملIsolation of a cytochrome aa(3) gene from Bradyrhizobium japonicum.
Bradyhizobium japonicum strain LO501 is a Tn5-induced mutant that does not express the terminal oxidase cytochrome aa(3) (cytochrome-c oxidase, EC 1.9.3.1). Two and one-half kilobase pairs of LO501 genomic DNA that flanks the transposon was isolated and used as a hybridization probe to obtain the wild-type gene from a cosmid library. Two subcloned fragments from two of the isolated cosmids were...
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ژورنال
عنوان ژورنال: Molecular Genetics and Genomics
سال: 2010
ISSN: 1617-4615,1617-4623
DOI: 10.1007/s00438-010-0547-2