Autoradiographic localization of adenosine uptake sites in rat brain using [3H]nitrobenzylthioinosine
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منابع مشابه
Autoradiographic localization of adenosine uptake sites in rat brain using [3H]nitrobenzylthioinosine.
The adenosine uptake site has been localized in rat brain by an in vitro light microscopic autoradiographic method, using [3H]nitrobenzylthioinosine ( [3H]NBI) as the probe. The binding characteristics of [3H]NBI on slide-mounted sections are comparable to those seen in studies performed on brain homogenates. A very high density of uptake sites occurs in the nucleus tractus solitarius, in the s...
متن کاملAdenosine uptake sites in rat brain: identification using [3H]nitrobenzylthioinosine and co-localization with adenosine deaminase.
The binding characteristics of [3H]nitrobenzylthioinosine ([3H]NBI) to rat brain membrane preparations was examined, and the autoradiographic distribution of this ligand in brain sections was compared with the immunohistochemical localization of adenosine deaminase (ADA). It was found that [3H]NBI labels sites for which adenosine has far higher affinity than do other nucleosides, that these sit...
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Relaxin is a member of the insulin family of polypeptide hormones and exerts its best understood actions in the mammalian reproductive system. Using a biologically active 32P-labeled human relaxin, we have previously shown by in vitro autoradiography specific relaxin binding sites in rat uterus, cervix, and brain tissues. Using the same approach, we describe here a detailed localization of huma...
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Adenosine (A1) receptor binding sites have been localized in rat brain by an in vitro light microscopic autoradiographic method. The binding of [3H]N6-cyclohexyladenosine to slide-mounted rat brain tissue sections has the characteristics of A1 receptors. It is saturable with high affinity and has appropriate pharmacology and stereospecificity. The highest densities of adenosine receptors occur ...
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ژورنال
عنوان ژورنال: The Journal of Neuroscience
سال: 1985
ISSN: 0270-6474,1529-2401
DOI: 10.1523/jneurosci.05-02-00544.1985