Automated measurement of amylase isoenzymes with 4-nitrophenyl-maltoheptaoside as substrate and use of a selective amylase inhibitor.
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Automated measurement of amylase isoenzymes with 4-nitrophenyl-maltoheptaoside as substrate and use of a selective amylase inhibitor.
We automated a kinetic procedure for determining amylase isoenzymes in serum and urine samples. We used 4-nitro-phenylmaltoheptaoside as substrate and a selective amylase inhibitor with the Abbott-VP bichromatic system. By use of the maximum differences between pancreatic (P) and salivary (S) amylase activities remaining after inhibition by the selective inhibitor and by use of the linear range...
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We evaluated the enzymic mechanism by which 3-keto butylidene-beta-2-chloro-4-nitrophenyl maltopentaoside (3KB-G5-CNP) serves as a substrate for serum pancreatic (p-) and salivary (s-) amylases. In aliquots of the reaction mixture, three kinds of beta-2-chloro-4-nitrophenyl oligosaccharides (glucose, maltoside, and maltotrioside) were separated from the substrate by high-performance liquid chro...
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A simple, rapid screening procedure for determining the relative amounts of pancreatic (P)- and salivary (S)-type amylase in serum is presented, involving incorporation of a selective inhibitor (from wheat-germ) into commercially available BMD Single-Vial Amylase and Beckman Enzymatic Amylase-DS procedures for manual and automated isoamylase measurements. Optimal concentrations of inhibitor inh...
متن کاملReference Ranges for ot-Amylase in Serum and Urine with 4?6-Ethylidene-(G7)-l-4-nitrophenyl-(Gl)-a5D-maltoheptaoside äs Substrate
Reference ranges for -amylase in serum, spontaneously voided urine, and 24h urine were determined, using 4,6-ethylidene-(G7)-l-4-nitrophenyl-(Gl)-a,£)-malthoheptaoside äs the Substrate (EPS method), at 25, 30, and 37 °C. The measured values were evaluated with and without the use of a factor which converts the results of the -amylase EPS method into values comparable to those obtained with the ...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1984
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/30.7.1219