Automated Extracellular Vesicles Extractor Based on Nanotechnology for High Throughput Sample Preparation and Phosphoproteomics Analysis

Extracellular vesicles (EVs) play important roles in the diagnosis and treatment of diseases because its lavish bioinformatic molecular contents. However, how to separate plentiful extracellular a short time with high consistency is still problem. Here, we’d like introduce an automated capturing machine named EVenrich (Extracellular Vesicles Enrichment) that helps realize EVs biosamples high-throughput manner. Incorporating EVtraps-a kind biological magnetic beads specifically, we were able isolate from up 48 urine samples 60 min, similar quality quantity superior stability manual capture. In clinical analysis, over 14000 peptide fragments 2000 proteins detected by capture, which was capture significantly ultracentrifugation. We further collected 21 patients negative prostate biopsy results confirmed cancer. Every three mixed into group make 7 control groups disease groups. After exosome extraction proteomics / phosphorylated found 211 overexpressed 187 peptides corresponding 40 as potential biomarkers for addition, qRT-PCR showed ratio miR-125b miR-145 could well distinguish cancer non-prostate patients, suggesting it be used marker