Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood
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چکیده
منابع مشابه
Automated enzymatic fluorometric method for the determination of pyruvic and lactic acids in blood.
A simple automated fluorometric method for the estimation of lactate and pyruvate is described. Analyses can be performed at 60/hour and can conveniently determine pyruvate and lactate in the ranges 0-5 mg. and 0-30 mg/100 ml. The methods give a high degree of analytical precision and good yields from recovery experiments.
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The accurate determination of pyruvic and lactic acids is a matter of considerable theoretical and practical importance. These acids play a role in the metabolism of carbohydrates, and they are found in increased concentration in the blood in conditions of thiamine deficiency. Recently we suggested (1) the determination of the ratio of lactic to pyruvic acid in the blood as a measure of oxidati...
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1N11 ANY clinical and experimental problems concerning anaerobic metabolism and the accumulation of an oxygen debt have recently been studied, using Huckabee’s concept of “excess lactate” (1). Colonmetric methods have most often been used to measure pyruvic and lactic acid in blood (2, 3). The estimation of these two metabolites by an enzymatic analysis has advantages-e.g., specificity and ease...
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A simple automated method for the determination of lactic dehydrogenase (LDH) activity in serum by the forward lactate pyruvate reaction is presented. Enzyme activity is determined by measuringthe fluorescence of DPNH formed after a fixed incubation time. Detailed studies of various parameters of the reaction, reproducibility, and recovery data, and a comparisonwith two manual methods for LDII ...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1968
ISSN: 0021-9746
DOI: 10.1136/jcp.21.2.171