Automated Determination of Heparin with Chromogenic Substrates
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چکیده
منابع مشابه
Automated determination of heparin with chromogenic substrates.
Spectrophotometric heparin assays which are based on the catalytic effect of heparin on either the inactivation of thrombin or that of factor Xa by antithrombin III, were adapted for use in a laboratory batch analyzer. Optimal conditions were determined for assays using the chromogenic substrates Chromozym-Th and S-2238 with thrombin, and S-2222 with factor Xa. Inactivation of the clotting enzy...
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Blood plasma contains a number of proteins capable of inhibiting the activation of plasminogen and the action of plasmin. Except for urokinase, the nature and action of plasminogen inhibitors are not yet fully understood. Inhibitors of plasmin, on the other hand, are fairly well defined and their clinical significance recognised. Although there are a number of plasmin inhibitors in plasma-for e...
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BACKGROUND Proteinase activities are often measured using chromogenic substrates that are much smaller than physiological substrates. METHODS The hydrodynamic size of macromolecular substrates (macrosubstrates) prepared by linking small chromogenic substrates to polyethylene glycol was determined by gel filtration. Efficiency of macrosubstrate cleavage by proteinases and alpha(2)-macroglobuli...
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The structure-activity relationship of dipetalogastin II, the strongest thrombin inhibitor isolated and cloned from the bug Dipetalogaster maximus, was examined by introducing gradual changes into the molecule by means of molecular biological methods. The effect upon its inhibition equilibrium constant was determined after each change by a chromogenic assay. This structural information was fund...
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ژورنال
عنوان ژورنال: Pathophysiology of Haemostasis and Thrombosis
سال: 1984
ISSN: 1424-8832,1424-8840
DOI: 10.1159/000215055