AtWuschel Promotes Formation of the Embryogenic Callus in Gossypium hirsutum

AtWuschel Promotes Formation of the Embryogenic Callus in Gossypium hirsutum

Upland cotton (Gossypium hirsutum) is one of the most recalcitrant species for in vitro plant regeneration through somatic embryogenesis. Callus from only a few cultivars can produce embryogenic callus (EC), but the mechanism is not well elucidated. Here we screened a cultivar, CRI24, with high efficiency of EC produce. The expression of genes relevant to EC production was analyzed between the ...

Polyamine and Its Metabolite H2O2 Play a Key Role in the Conversion of Embryogenic Callus into Somatic Embryos in Upland Cotton (Gossypium hirsutum L.)

The objective of this study was to increase understanding about the mechanism by which polyamines (PAs) promote the conversion of embryogenic calli (EC) into somatic embryos in cotton (Gossypium hirsutum L.). We measured the levels of endogenous PAs and H2O2, quantified the expression levels of genes involved in the PAs pathway at various stages of cotton somatic embryogenesis (SE), and investi...

In vitro Plant Regeneration from the Callus of Shoot Tips in Cotton (Gossypium hirsutum L. cv. SVPR 2)

A high efficiency shoot organogenesis from callus cultures and plant establishment protocol has been developed for cotton (Gossypium hirsutum L. cv SVPR-2) through shoot tip. After 6 weeks of culture, green-compact-nodular organogenic callus induction was observed on the medium fortified with (MS) basal salts, B5 vitamins, 2.0 mgl-1 of benzylaminopurine (BAP), 1.0 mgl-1 kinetin (KN), 30 gl-1 gl...

The biology and ecology of cotton (Gossypium hirsutum) in Australia

The Anomalous Behavior of the Ghost Spot of GOSSYPIUM ANOMALUM in Amphidiploid GOSSYPIUM HIRSUTUM.

ILD Gossypium anomalum Wawrs. and Peyr. among Gossypium species whas anomalous petal spot inheritance. Its petal spot involves two independently inherited genes; a ghost spot gene interacts with a spotless gene making a large anthocyanin area at the base of a petal (SILOW 1941). In the two cultivated Asiatic diploid species red petal spot involves three closely linked genesmargin. ghost spot, a...