Astragaloside IV inhibits the proliferation and migration of lens epithelial cells induced by TGF-β through the Jagged-1/Notch pathway

Aberrant proliferation and migration of residual lens epithelial cells (LECs) is the main cause posterior capsule opacification (PCO) after cataract surgery. The purpose this study was to confirm protective effects Astragaloside IV (AST) in transforming growth factor beta (TGF-β)-induced LECs. Human LECs, SRA01/04, were used study. MTT assay EdU perform cell viability proliferation, respectively. cycle determined using flow cytometry. Cell invasion transwell wound healing assay, Protein expression examined western blotting. Type I collagen (Col IV) fibronectin (FN) also detected immunofluorescence (IF). viability, suppressed by AST a concentration-dependent manner TGF-β-treated stopped at G2 phase manner. Expression Cyclin B1 CDK1 FN, Col I, IV, α-SMA (α-smooth muscle actin), Snail Slug downregulated inhibited protein Jagged-1, Notch1, Notch2, Notch3, Hes-1 Hey-1. Taken together, data from demonstrated that LEC invasion, as well fibroblastic differentiation epithelial-mesenchymal transition process through inhibition Jagged-1/Notch pathway, providing potential supplemental treatment for PCO.