Associations of antibodies to benzo[a]pyrene, estradiol and progesterone with estrogen receptor phenotype in breast cancer tissue

Aim. To reveal the associations of IgA antibodies to benzo[a]pyrene, estradiol and progesterone (IgA-Bp, IgA-Es, IgA-Pg) with conversion estrogen-receptor positive (ER+) into negative (ER-) tumors during breast cancer progression. Materials Methods. Having collected serum samples from 338 healthy volunteers 1407 patients, we have profiled them for IgA-Bp, IgA-Pg by means enzyme-linked immunosorbent assay. Conjugates bovine albumin Bp, Es Pg were used as adsorbed antigens anti-human horseradish peroxidase-conjugated detection specific antigen-bound antibodies. Individual IgA-Bp/IgA-Pg IgA-Es/IgA-Pg ratios calculated. Estrogen receptor phenotype was determined using immunohistochemistry. Results. Low (≤ 1) in combination low indicative protective immunophenotype more frequently revealed women (43.8%) comparison stage 1 patients ER+ (12.9%) ER- (23.9%) tumors. High (>1) high suggestive pro-carcinogenic immunological less often detected (27.5%) compared (65.5%) (58.7%) Prevalence phenotypes significantly differed 1breast tumor (p = 0.017). prevalent at II-IV stages (25.6%) than (16.3%). Conversion reflecting progression characteristic (p<0.0001). Conclusion. Detection against may be applied a risk marker development