Association with AflR in Endosomes Reveals New Functions for AflJ in Aflatoxin Biosynthesis

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Association with AflR in Endosomes Reveals New Functions for AflJ in Aflatoxin Biosynthesis

Aflatoxins are the most potent naturally occurring carcinogens of fungal origin. Biosynthesis of aflatoxin involves the coordinated expression of more than 25 genes. The function of one gene in the aflatoxin gene cluster, aflJ, is not entirely understood but, because previous studies demonstrated a physical interaction between the Zn2Cys6 transcription factor AflR and AflJ, AflJ was proposed to...

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Characterization of aflJ, a gene required for conversion of pathway intermediates to aflatoxin.

The genes encoding the aflatoxin biosynthetic pathway enzymes have been localized as a cluster to a 75-kb DNA fragment. The enzymatic functions of the products of most of the genes in the cluster are known, but there are a few genes that have not yet been characterized. We report here the characterization of one of these genes, a gene designated aflJ. This gene resides in the cluster adjacent t...

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Expression of genes of the aflatoxin biosynthetic pathway in Aspergillus flavus isolates from keratitis

PURPOSE To document transcriptional activation (expression) of key aflatoxin biosynthetic pathway genes in corneal isolates of Aspergillus flavus. METHODS The expression of certain regulatory (aflatoxin regulatory [aflR] and aflatoxin J [aflJ]) and structural (polyketide synthase acetate [pksA] and norsolonic acid-1 [nor-1]) genes in four corneal A. flavus isolates was evaluated by reverse tr...

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Aflatoxin biosynthesis.

Aflatoxins are toxic and extremely carcinogenic natural secondary metabolites produced primarily by the fungi Aspergillus flavus and Aspergillus parasiticus. The biosynthesis of aflatoxins is a complex process involving multi-enzymatic reactions. Genetic studies of the molecular mechanism of aflatoxin B1 biosynthesis have identified an aflatoxin pathway gene cluster of 70 kilobase pairs in leng...

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ژورنال

عنوان ژورنال: Toxins

سال: 2012

ISSN: 2072-6651

DOI: 10.3390/toxins4121582