Assessment of peanut allergen Ara h1 in processed foods using a SWCNTs-based nanobiosensor
نویسندگان
چکیده
منابع مشابه
Peanut allergen (Ara h 1) detection in foods containing chocolate.
Inadvertent exposure to peanut in foods poses health risks for peanut-allergic individuals that can be reduced by improving detection systems for allergen contaminants in food products and manufacturing processes. Detection of peanut in chocolate has been especially difficult. We report the optimization of conditions for measuring a major peanut allergen, Ara h 1, in chocolate with the use of a...
متن کاملNanopore immunosensor for peanut protein Ara h1
A nanopore immunosensor is demonstrated for peanut protein Ara h1, a common food allergen. This biosensor is constructed by immobilizing the antibody to peanut protein Ara h1 within Au-coated pores of commercial nanoporous polycarbonate membranes. Peanut protein Ara h1 is detected as the change in the pore conductivity as the pores are partially obscured by antigen binding. Control experimentsw...
متن کاملDevelopment of a Monoclonal Antibody-Based Sandwich ELISA for Peanut Allergen Ara h 1 in Food
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of dete...
متن کاملThe individual role of peanut proteins Ara h1, 2, 3 and 6 in peanut allergy
One of the major allergenic food or food constituent is peanut, in which a number of allergenic proteins have been described (Ara h1-9). Until now, the relative contribution of the individual peanut allergens to clinical food allergic responses is not known. Therefore, the present studies aimed to elucidate the relative contribution of Ara h1, 2, 3, and 6 to peanut allergy in a mouse model. For...
متن کاملRecombinant peanut allergen Ara h I expression and IgE binding in patients with peanut hypersensitivity.
Peanut allergy is a significant health problem because of the frequency, the potential severity, and the chronicity of the allergic sensitivity. Serum IgE from patients with documented peanut hypersensitivity reactions and a peanut cDNA expression library were used to identify clones that encode peanut allergens. One of the major peanut allergens, Ara h I, was selected from these clones using A...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 2018
ISSN: 0916-8451,1347-6947
DOI: 10.1080/09168451.2018.1453295