Assessment of genetic fidelity of micropropagated date palm (Phoenix dactylifera L.) plants by RAPD and ISSR markers assay
نویسندگان
چکیده
منابع مشابه
Genetic diversity analysis of date palm (Phoenix dactylifera L.) in the Kutch region of India using RAPD and ISSR markers
First line in abstract should be Date palm is an important fruit crop of the arid and semiarid region. In the present investigation two types of DNA based marker RAPD and ISSR were used to characterize 8 date palm genotypes grown in the Kutch region. Amplification of genomic DNA of 8 genotypes using 13 RAPD analyses yielded 88 fragments, of which 35 were polymorphic, with an average of 2.69 pol...
متن کاملNew microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)
New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A to...
متن کاملIdentification of Genetic Variation of Male and Female Date Palm (Phoenix dactylifera L.) Cultivars Using Morphological and Molecular Markers
Date palm (Phoenix dactylifera L.) is a monocot, dioecious and high longevity tropical plant greatly considered due to its high economic importance in Middle East. This investigation was carried out to detect genetic relations among 34 cultivars of Iranian date palm, both males and females, using ISSR markers. Accordingly, total DNA was extracted using CTAB method and was subjected to PCR ampli...
متن کاملGenome sequence of the date palm Phoenix dactylifera L
Date palm (Phoenix dactylifera L.) is a cultivated woody plant species with agricultural and economic importance. Here we report a genome assembly for an elite variety (Khalas), which is 605.4 Mb in size and covers >90% of the genome (~671 Mb) and >96% of its genes (~41,660 genes). Genomic sequence analysis demonstrates that P. dactylifera experienced a clear genome-wide duplication after eithe...
متن کاملDirect Organogenesis of Date Palm (Phoenix dactylifera L.) for Propagation of True-to-Type Plants
RAPD analysis This study aimed to develop an efficient method for micrpropagation of true-to-type date palm plants through direct organogenesis. Nodular cultures were obtained from shoot tips on MS medium plus 2 mg/l 2ip and 1 mg/l NAA. Among combinations of 2ip and 2,4-D added to culture medium for direct shoot buds proliferation, 5 mg/l 2ip alone gave the highest organogenesis frequency. For ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Physiology and Molecular Biology of Plants
سال: 2010
ISSN: 0971-5894,0974-0430
DOI: 10.1007/s12298-010-0023-9