Assessment of cytotoxicity biomarkers on the microalga Chlamydomonas reinhardtii exposed to emerging and priority pollutants
نویسندگان
چکیده
Contamination of aquatic ecosystems linked to anthropogenic activity is currently a major concern; therefore, ecotoxicological studies are needed assess its effect on organisms. The main objective this study was investigate the effects different pollutants microalgae in search sensitive biomarkers that can promote common cytotoxic response regardless contaminant. Cultures freshwater microalga Chlamydomonas reinhardtii were exposed for 24 h four chemicals, three emerging (benzophenone-3, bisphenol A and oxytetracycline) one priority substance (atrazine). cytometric panel carried out toxicity including cellular growth, inherent cell properties, viability, vitality, cytoplasmic membrane potential ROS levels. Lipid peroxidation, photosynthetic efficiency transcriptional responses photosynthesis- oxidative stress-related genes using RT-qPCR also studied. Some showed similar pattern; decrease growth rate, vitality an increase autofluorescence number cells with depolarised found all chemicals tested. However, ATZ OTC provoked size, whereas BP-3 BPA caused intracellular complexity levels viability. Assayed generally promoted overexpression related antioxidant defence system subexpression photosynthesis-related genes. In addition traditional endpoint, gene expression catalase, glutathione peroxidase Fe-superoxide dismutase significantly affected tested, showing response. Among tested substances, strongest alterations microalga, pointing some contaminants could be more harmful organisms than pollutants.
منابع مشابه
Title: Acute effects of a prooxidant herbicide on the microalga Chlamydomonas reinhardtii: screening cytotoxicity and genotoxicity
متن کامل
Monitoring Autophagy in the Model Green Microalga Chlamydomonas reinhardtii
Autophagy is an intracellular catabolic system that delivers cytoplasmic constituents and organelles in the vacuole. This degradative process is mediated by a group of proteins coded by autophagy-related (ATG) genes that are widely conserved from yeasts to plants and mammals. Homologs of ATG genes have been also identified in algal genomes including the unicellular model green alga Chlamydomona...
متن کاملDevelopment of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii
BACKGROUND Oils produced by microalgae are precursors to biodiesel. To achieve a profitable production of biodiesel from microalgae, identification of factors governing oil synthesis and turnover is desirable. The green microalga Chlamydomonas reinhardtii is amenable to genetic analyses and has recently emerged as a model to study oil metabolism. However, a detailed method to isolate various ty...
متن کاملGrowth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii
Background Individual nutrient depletion is widely used to induce lipid accumulation in microalgae, which also causes cell growth inhibition and decreases the total biomass. Thus, improving the lipid accumulation without biomass loss in the nutrient deficiency cells becomes a potential cost-effective treatment for cheaper biofuels. Methods In this study, the effects of different nutritional c...
متن کاملon the relationship between self- regulated learning strategies use and willingness to communicate in the context of writing
این تحقیق به منظور بررسی رابطه بین میزان استراتژیهای خود-تنظیم شده یادگیری و تمایل به ایجاد ارتباط دانشجویان زبان انگلیسی انجام شده است.علاوه بر این،روابط و کنش های موجود بین ریزسنجه های استراتژیهای خود-تنظیم شده یادگُیری ، مهارت نگارش و تمایل به برقراری ارتباط و همچنین تاٍثیرجنسیت دانشجویان زبان انگلیسی در استراتژیهای خود-تنظیم شده یادگیری و تمایل به برقراری ارتباط آنها مورد بررسی قرار گرفته شد.
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Ecotoxicology and Environmental Safety
سال: 2021
ISSN: ['0147-6513', '1090-2414']
DOI: https://doi.org/10.1016/j.ecoenv.2020.111646