Assessing the Efficiency of RNA Interference for Maize Functional Genomics
نویسندگان
چکیده
منابع مشابه
Assessing the efficiency of RNA interference for maize functional genomics.
A large-scale functional genomics project was initiated to study the function of chromatin-related genes in maize (Zea mays). Transgenic lines containing short gene segments in inverted repeat orientation designed to reduce expression of target genes by RNA interference (RNAi) were isolated, propagated, and analyzed in a variety of assays. Analysis of the selectable marker expression over multi...
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RNA interference (RNAi) is the sequence-specific gene silencing induced by double-stranded RNA (dsRNA). Being a highly specific and efficient knockdown technique, RNAi not only provides a powerful tool for functional genomics but also holds a promise for gene therapy. The key player in RNAi is small RNA (approximately 22-nt) termed siRNA. Small RNAs are involved not only in RNAi but also in bas...
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A remarkable revolution is occurring in biomedical science that is likely to have profound implications for the way we treat human diseases in the future. In the past 2 years, we have learned that introduction of short double-stranded RNA (dsRNA) molecules into vertebrate cells can silence any gene homologous to the dsRNA. This process is called RNA interference (RNAi) and is based on the abili...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 2007
ISSN: 1532-2548
DOI: 10.1104/pp.106.094334