منابع مشابه
Improved Method of Phage Assay by a Plaque Count Method on Microscope Slides in Trays.
' Supported by grant no. E-2379 from the National Institutes of Health, U. S. Public Health Service. Since then, the Jones-Krueger method has been further simplified by placing a large number of slides on moistened blotters in flat trays which are then covered with glass sheets to retain moisture. Using trays 33.7 cm long, 23.5 cm wide, 1.3 cm high, the authors have routinely placed 32 slides p...
متن کاملA hemolytic plaque assay for activated murine T cells
In an earlier report, it was shown that murine spleen cells cultured with concanavalin A (Con A) released into the culture supernatants helper and suppressor substances for antibody production. The present communication describes the production of rabbit antisera against culture supernates from Con A-activated spleen cells and their use in a plaque assay for mitogen-activated T cells. The plaqu...
متن کاملHemolytic Plaque Assay
sions of new or recrudescent acute synovitis. RF-PFC were observed to disappear from the peripheral circulation and the bone marrow during therapy with cytotoxic drugs. The data are consistent with the hypothesis that the appearance of RF-PFC in the peripheral blood represents an anamnestic response to transiently appearing antigen. The nature of the antigen is not specified. The bone marrow ma...
متن کاملDetection of equine immunoglobulin-secreting cells by a plaque assay.
A protein A-hemolytic plaque assay was applied to detect immunoglobulin (Ig)-producing cells in horse peripheral blood, using pokeweed mitogen as a B lymphocyte activator. A maximum number of Ig-secreting cells was obtained when horse peripheral blood lymphocytes were cultured in a medium containing horse serum. The number of Ig-secreting cells in young horses (2 years old) was lower than that ...
متن کاملPlaque Assay for Murine Norovirus
Murine norovirus (MNV) is the only member of the Norovirus genus that efficiently grows in tissue culture. Cell lysis and cytopathic effect (CPE) are observed during MNV-1 infection of murine dendritic cells or macrophages. This property of MNV-1 can be used to quantify the number of infectious particles in a given sample by performing a plaque assay. The plaque assay relies on the ability of M...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1964
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.87.5.1177-1179.1964