Assay for quantitative determination of CYP1A1 enzyme activity using 7-Ethoxyresorufin as standard substrate (EROD assay)
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ENZYME-LINKED IMMUNOSORBENT ASSAY OF NEOPTERIN USING PENICILLINASE AS LABEL
An enzyme-linked immunosorbent assay for neopterin using penicillinase as marker enzyme is reported here by polyclonal antibodies against neopterin conjugated to bovine serum albumin which were raised in rabbits. Immunoglobulin fractions were purified and coated on wells of microtiter plates. A chain heterology was introduced in neopterin derivative and conjugated to penicillinase. The assa...
متن کاملBiological Activity Analysis of Native and Recombinant Streptokinase Using Clot Lysis and Chromogenic Substrate Assay
Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...
متن کاملBiological Activity Analysis of Native and Recombinant Streptokinase Using Clot Lysis and Chromogenic Substrate Assay
Determination of streptokinase activity is usually accomplished through two assay methods: a) Clot lysis, b) Chromogenic substrate assay. In this study the biological activity of two streptokinase products, namely Streptase®, which is a native product and Heberkinasa®, which is a recombinant product, was determined against the third international reference standard using the two forementioned a...
متن کاملA Heterologous Enzyme Linked Immunosorbant Assay of Morphine Using Penicillinase as Label
A rapid, sensitive, specific and high through-put enzyme-linked immunosorbant assay (ELISA) methodfor determination of morphine in urine samples using penicillinase as label enzyme has been developed. Noextraction or chromatography was included in this assay procedure. Immunoglobulin (Ig) purified polyclonalanti-bodies against a C6-hemisuccinate derivative of morphine (M-C6-HS...
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ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2014
ISSN: 2043-0116
DOI: 10.1038/protex.2014.043