AS-IV enhances the antitumor effects of propofol in NSCLC cells by inhibiting autophagy

Abstract Non-small cell lung cancer (NSCLC) is one of the most lethal malignant tumors. It has been shown that general anesthetic agents, propofol and astragaloside IV (AS-IV) both exert antitumor effects in NSCLC. However, combination with AS-IV NSCLC remain unclear. Cell counting kit-8, EdU Transwell assays were performed to evaluate viability, proliferation, migration. apoptosis autophagy observed by flow cytometric analysis TUNEL LC3 staining, respectively. notably enhanced anti-proliferative, pro-apoptotic, anti-migratory properties cells. Moreover, remarkably facilitated anti-autophagy effect cells downregulating LC3, Beclin 1, ATG5. Significantly, pro-apoptotic ability AS-IV/propofol was further inhibitor 3-MA, suggesting plays a tumor-promoting role Collectively, could facilitate abilities inhibiting autophagy. These findings may be beneficial for future studies on use treatment