Artificial Meiotic Arrest and Parthenogenetic Activation of Buffalo Oocytes Using Cycloheximide
نویسندگان
چکیده
منابع مشابه
Effects of cysteamine during in vitro maturation on viability and meiotic competence of vitrified buffalo oocytes
The aims of the present study were to assess the effects of cysteamine as an anti-oxidant on the rate of in vitro maturation (IVM) of buffalo oocytes (experiment 1), and their viability and nuclear status following vitrification (experiment 2). Immature oocytes with compact cumulus cells obtained from the ovaries of slaughtered animals were harvested and then cultured in the maturation medium w...
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The aim of the study was to compare the parthenogenetic activation and in vitro fertilization (IVF) of in vitro matured caprine oocytes. A total of 881 cumulus-oocyte complexes (COC’s) were collected from 243 ovaries. Oocytes were matured in TCM-199 medium containing eCG (20 IU/ml), hCG (20 IUμg/ml), oestradiol-17β (1 μg/ml), BSA embryo tested (3 mg/ml) supplemented with 10% fetal bovine serum ...
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Background: It is a common practice to rest vitrified-warmed matured oocytes for 1-3 hours, as a treatment to recover spindle and cytoskeleton, before commencing a further treatment. Vitrified-warmed matured oocytes, however, are very sensitive and may resume meiosis spontaneously during this recommended rest time. Therefore, the aim of this study was to assess spindle and chromosome status as ...
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In mammals, following implantation of the embryo, a small number of cells from the epiblast eventually become the precursors of primordial germ cells (reviewed by Edson et al., 2009). After mitotic proliferation of the primordial germ cells, migration to the genital ridges and rapid proliferation again, meiosis is initiated in the oogonia at about day 13 of development in mice, at day 82 in bov...
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Purpose: Parthenogenetic activation is among the crucial steps determining successful development of mammalian cloned embryos. This study, therefore, was conducted to evaluate the efficiency of a novel combined electrical-chemical artificial activation method for bovine cloning. Materials and Methods: In vitro matured bovine oocytes than were initially exposed to electrical pulse, used for cel...
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ژورنال
عنوان ژورنال: CYTOLOGIA
سال: 2007
ISSN: 0011-4545,1348-7019
DOI: 10.1508/cytologia.72.173