Artemia as a possible vector for Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus transmission (XSV) to Macrobrachium rosenbergii post-larvae
نویسندگان
چکیده
منابع مشابه
Monoclonal antibodies against extra small virus show that it co-localizes with Macrobrachium rosenbergii nodavirus.
The capsid protein (CP) gene of extra small virus (XSV) expressed in Escherichia coli as a 42 kDa glutathione S-transferase (GST)-fusion protein (GST-XCP) or a 20 kDa His6-fusion protein (His6-XCP) were purified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), combined, and used to immunize Swiss mice to produce monoclonal antibodies (MAbs). Using dot blot, Western blot,...
متن کاملTracking the virus-like particles of Macrobrachium rosenbergii nodavirus in insect cells
Macrobrachium rosenbergii nodavirus (MrNv) poses a major threat to the prawn industry. Currently, no effective vaccine and treatment are available to prevent the spread of MrNv. Its infection mechanism and localisation in a host cell are also not well characterised. The MrNv capsid protein (MrNvc) produced in Escherichia coli self-assembled into virus-like particles (VLPs) resembling the native...
متن کاملTolerance of Macrobrachium rosenbergii to white spot syndrome virus
The susceptibility of Macrobrachium idella, M. lamerrae and M. rosenbergii to white spot Ž . syndrome virus WSSV was tested by immersion challenge, oral route and intramuscular injection. Their susceptibility to WSSV was compared with that of Penaeus indicus and P. monodon. The WSSV caused 43.3% and 53.3% mortality in M. lamerrae and M. idella, respectively, by immersion method and 53.3% and 66...
متن کاملProduction of monoclonal antibodies specific to Macrobrachium rosenbergii nodavirus using recombinant capsid protein.
The gene encoding the capsid protein of Macrobrachium rosenbergii nodavirus (MrNV) was cloned into pGEX-6P-1 expression vector and then transformed into the Escherichia coli strain BL21. After induction, capsid protein-glutathione-S-transferase (GST-MrNV; 64 kDa) was produced. The recombinant protein was separated using SDS-PAGE, excised from the gel, electro-eluted and then used for immunizati...
متن کاملMacrobrachium rosenbergii nodavirus infection in a giant freshwater prawn hatchery in Indonesia
A pathogen of giant freshwater prawn, Macrobrachium rosenbergii, was recently recorded in a hatchery in Yogyakarta. The clinical symptom in post-larvae (PL) was a whitish appearance of the muscles in the tail. Histological examination revealed myonecrosis with massive infiltration of myonuclei and hemocytes. RT-PCR products of 850 bp were obtained when using RNA from diseased PL as a template. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Diseases of Aquatic Organisms
سال: 2006
ISSN: 0177-5103,1616-1580
DOI: 10.3354/dao070161