منابع مشابه
Approaches to Analyze Protein-Protein Interactions of Membrane Proteins
It can be estimated that most membrane proteins function in complexes (Fig. 1) (Daley 2008). Protein-protein interactions (PPIs) within these complexes can either be direct (primary interaction) or indirect (secondary interaction). Direct interactions occur either by homooligomerisation as determined for bacterial two-component systems (Gao and Stock 2009) (Fig. 1A) or by hetero-oligomerisation...
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The availability of complete, annotated genome sequences for a variety of eukaryotic organisms has paved the way for a paradigm shift in biomedical research from the 'one gene-one hypothesis' approach to more global, systematic strategies that analyse genes or proteins on a genome- and proteome-wide scale. One daunting task in the post-genome era is to determine how the complement of expressed ...
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In the present article, we describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of Saccharomyces cerevisiae, showing that the majority of proteins are part of complexes, and that complexes typically consist of a core to which are b...
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ژورنال
عنوان ژورنال: The Scientific World JOURNAL
سال: 2002
ISSN: 1537-744X
DOI: 10.1100/tsw.2002.44