Application of Temperature-Gradient Gel Electrophoresis for Detection of Prion Protein Gene Polymorphisms in Polish Świniarka Sheep
نویسندگان
چکیده
منابع مشابه
Prion protein (PrP) gene polymorphisms and breeding for resistance to scrapie in Polish Merino sheep
Genetic susceptibility or resistance of sheep to scrapie is associated with polymorphisms particularly in the three codons 136, 154 and 171 of the ovine prion protein (PrP) gene. The aim of the study was to reveal the PrP haplotype and genotype distribution in the Polish Merino breed and to make a forecast of the application of a scrapie resistance breeding programme for this breed. PCR-RFLP an...
متن کامل: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
Prion protein gene (PrP) polymorphisms in healthy sheep in Turkey.
Scrapie, a transmissible spongiform encephalopathy (TSE) or prion disease, is a fatal, neurodegenerative disease in sheep and goats. This disease has been known in Europe for more than 250 years. Susceptibility to scrapie is associated with polymorphisms in the sheep prion protein gene (PrP) gene. In sheep, polymorphism in the PrP gene has been identified at a number of codons, and polymorphism...
متن کاملextremal region detection guided by maxima of gradient magnitude
a problem of computer vision applications is to detect regions of interest under dif- ferent imaging conditions. the state-of-the-art maximally stable extremal regions (mser) detects affine covariant regions by applying all possible thresholds on the input image, and through three main steps including: 1) making a component tree of extremal regions’ evolution (enumeration), 2) obtaining region ...
Application of temperature-gradient gel electrophoresis in taxonomy of coryneform bacteria.
Strains belonging to the Gram-positive coryneform soil bacteria were screened genotypically by temperature-gradient gel electrophoresis (TGGE). This method allows the sequence-specific separation of amplified fragments of 16S rRNA genes. A total of 115 reference strains representing the majority of the species of the genera Aeromicrobium, Agromyces, Arthrobacter, Aureobacterium, Cellulomonas, C...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Veterinary Diagnostic Investigation
سال: 2006
ISSN: 1040-6387,1943-4936
DOI: 10.1177/104063870601800307