Application of hanging drop technique for stem cell differentiation and cytotoxicity studies
نویسندگان
چکیده
منابع مشابه
Etiology and Use of the “Hanging Drop” Technique: A Review
Background. The hanging drop (HD) technique presumably relies on the presence of subatmospheric epidural pressure. It is not clear whether this negative pressure is intrinsic or an artifact and how it is affected by body position. There are few data to indicate how often HD is currently being used. Methods. We identified studies that measured subatmospheric pressures and looked at the effect of...
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The hanging drop tissue culture is a technique utilized in embryology [4] and other fields to allow growth that would otherwise be restricted by the flat plane of culture dishes and also to minimize the surface area to volume ratio, slowing evaporation. The classic hanging drop culture is a small drop of liquid, such as plasma or some other media allowing tissue growth, suspended from an invert...
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In this exercise you will learn to make hanging drop slides and then use this technique to observe the motility of living bacteria. Motility is an important characteristic used to identify microorganisms. Three bacteria will be used which vary in size, shape and arrangement of flagella, and types of motion. Specifically you will observe Pseudomonas aeruginosa, a monotrichous bacterium that exhi...
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Purpose: Differentiation of mouse embryonic stem cells into Insulin secreting endocrine cells. Materials and Methods: In this study, Royan B1 mouse embryonic stem cell (derived from C57BL/6 mouse) were used. In directed differentiation method, embryonicstem cells after embryoid bodies formation were differentiated into insulin secreting cells. Nestin positive cells were obtained after culture ...
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abstract: mineral scaling in oil and gas production equipment is one of the most important problem that occurs while water injection and it has been recognized to be a major operational problem. the incompatibility between injected and formation waters may result in inorganic scale precipitation in the equipment and reservoir and then reduction of oil production rate and water injection rate. ...
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ژورنال
عنوان ژورنال: Cytotechnology
سال: 2006
ISSN: 0920-9069,1573-0778
DOI: 10.1007/s10616-006-9001-z