Application Marker-Assisted Selection (MAS) and Multiplex PCR Reactions in Resistance Breeding of Maize (Zea mays L.)

Cultivated maize (Zea mays L.) is the oldest and one of most important crop species in world. Changing climatic conditions recent years, warm weather, expansion acreage intensification cultivation have resulted an increase threat posed by diseases caused by, among others, Fusarium fungi. Breeding success all plant determined access to starting materials with possible high genetic diversity also terms disease resistance. Identification parental combinations that produce offspring are high-yielding resistant Fusarium, other diseases, costliest steps breeding programs. We used lines which, as a result five-year field observations, were divided into susceptible F. verticillioides. It known resistance fusarium trait strongly dependent on environmental conditions. Due fact years observation degree infestation hot dry, some could from favorable In view above, aim this study was analyze basis these correlate molecular analyses observations. Comprehensive will allow selection reference be and, at same time, pyramidized genes. Such can for crossbreeding obtain fusarium-resistant varieties. addition, attempt made develop Multiplex PCR faster identification analyzed markers. As analyses, it found studied correlated number markers identified them. Both laboratory shown best line source genes 25. has level 8–9 nine-point COBORU scale. line, 10 out 12 (SSR 85, Bngl 1063, 1740, Umc 2082, 1621, 2059, 2013, SSR 93, 105, STS 03) related genes, which may reason such pathogen. Similarly, 9 35 03). This however, characterized slightly lower 7–8. Line 254 turned least resistant, 4–5, 5. Lines numbered 25 successfully