Antisperm antibodies modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm
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چکیده
منابع مشابه
Antisperm antibodies modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm.
BACKGROUND The hypo-osmotic swelling (HOS) test evaluates the ability of the functional sperm plasma membrane to stretch following cell swelling when exposed to hypo-osmotic solutions. Sperm samples with low HOS scores show low fertilization and pregnancy rates during assisted reproductive techniques, though data are controversial. The aim of this study was to compare the results of the HOS tes...
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We have successfully applied SDS (sodium dodecyl sulfate) gel/protein blot radioimmunobinding method to identify the molecular size of sperm antigens that elicit antisperm antibodies from patients with unexplained infertility. Following the transfer of renatured proteins from SDS gel of human sperm extract onto nitrocellulose strips, the radioimmunobinding was performed by incubating the strips...
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Mammalian spermatozoa must undergo capacitation and acrosome reaction before fertilization. To date, the precise mechanisms regulating these complex processes are not well understood but it is generally agreed that they involve an influx of calcium from the extracellular space through, as yet, poorly characterized plasma membrane pathways. Here we present evidence for a novel mechanism to incre...
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The present study was taken to characterize molecular weights of sperm specific polypeptides antigenic to rabbits and calf with the aim to assess their immunoreactivity with IgG antibodies in sera from immuno-infertile cows. Seropositivity for antisperm IgG antibodies in 75 repeat breeder and 15 pregnant control cattle was tested by cellular ELISA using washed spermatozoa antigen from 4 bulls. ...
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background: exposure to arsenic is associated with impairment of male reproductive function by inducing oxidative stress. silymarin with an antioxidant property scavenges free radicals. objective: the aim of this study was to investigate if silymarin can prevent the adverse effects of sodium arsenite on ram sperm plasma membrane and acrosome integrity. materials and methods: ram epidydimal sper...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 2004
ISSN: 1460-2350
DOI: 10.1093/humrep/deh317