Antisense ACC Oxidase RNA Delays Carnation Petal Senescence

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Antisense ACC Oxidase RNA Delays Carnation Petal Senescence

The phytohormone ethylene is essential for senescence in many fruits and flowers. In the biosynthesis of ethylene in plants, the conversion of S-adenosyl methionine (SAM) to 1-aminocyclopropane1-carboxylic acid (ACC) and the conversion of ACC to ethylene are catalyzed by ACC synthase (ACS) and ACC oxidase (ACO), respectively (Adams and Yang, 1979). Using a cDNA clone representing carnation ACO ...

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Reversible inhibition of ethylene action and interruption of petal senescence in carnation flowers by norbornadiene.

The inhibitory effects of the cyclic olefin 2,5-norbornadiene (NBD) on ethylene action were tested in carnation (Dianthus caryophyllus L. cv White Sim) flowers. Treatment of flowers at anthesis with ethylene in the presence of 500 microliters per liter NBD increased the concentration of ethylene required to elicit a response (petal senescence), indicating that NBD behaves as a competitive inhib...

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Is a cysteine proteinase inhibitor involved in the regulation of petal wilting in senescing carnation (Dianthus caryophyllus L.) flowers?

Senescence of carnation petals is accompanied by autocatalytic ethylene production and wilting of the petals; the former is caused by the expression of 1-aminocyclopropane-1-carboxylate (ACC) synthase and ACC oxidase genes and the latter is related to the expression of a cysteine proteinase (CPase) gene. CPase is probably responsible for the degradation of proteins, leading to the decomposition...

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Crystal structure of the petal death protein from carnation flower.

Expression of the PSR132 protein from Dianthus caryophyllus (carnation, clover pink) is induced in response to ethylene production associated with petal senescence, and thus the protein is named petal death protein (PDP). Recent work has established that despite the annotation of PDP in sequence databases as carboxyphosphoenolpyruvate mutase, the enzyme is actually a C-C bond cleaving lyase exh...

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Role of cytokinins in carnation flower senescence.

Stem and leaf tissues of carnation (Dianthus caryophyllus) plants appear to contain a natural antisenescence factor since removal of most of these tissues from cut carnation flowers hastened their senescence. However, kinetin (5-10 mug/ml) significantly delayed senescence of flowers with stem and leaf tissues removed. In addition, the life span of cut flowers with intact (30-cm) stems was incre...

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ژورنال

عنوان ژورنال: HortScience

سال: 1995

ISSN: 0018-5345,2327-9834

DOI: 10.21273/hortsci.30.5.970