Antiproliferative Effect of Polysaccharide Fraction of Astragalus Ovinus Against MCF-7 Cancer Cell Line

Background: The effect of Astragalus has been recognized in traditional Chinese medicine, and the roots are believed to have anti-cancer properties. This study investigated effects ethanolic extract polysaccharide fraction ovinus on MCF7 cell line. Methods: We used MTT assay evaluate cytotoxicity A. roots. status cycle apoptosis were examined, using flow cytometry. gene expressions related extrinsic intrinsic apoptotic pathways (caspases 8 & 9) also examined by real-time polymerase chain reaction (PCR). Results: data showed that inhibited proliferation cancer cells a dose-dependent manner. IC50 Cisplatin , polysaccharides 22.42, 560.9, 961.2 µg/ml, respectively. Also, analysis fractions arrested G1 phase. Examination treatment with either or induced MCF-7 cells. expression caspases 9 genes was after exposure cisplatin (PFA). However, only caspase-8 expression. Conclusion: results confirmed caused anti-proliferative Therefore, it can be concluded potential therapeutic against human breast