Antiproliferative activity of extract from in vitro callus cultures of Astragalus vesicarius ssp. carniolicus (A. Kern.) Chater
نویسندگان
چکیده
Five isoflavonoids, i.e. 5-hydroxy-7-methoxy-2’, 5’-dihydroxyisoflavone (AV4), 5, 7-dihydroxy-4’-methoxyisoflavone (AV6), 7-methoxy-5-hydroxy-4’-methoxy-2’-hydroxyisoflavone (AV7), 8-pregnyl genistein (AV9), 5,7-dihydroxy-8-pregnyl-4’-methoxy-2’-hydroxyisoflavone (AV10) and one coumarochromone – sophorophenolone (AV8) were isolated from EtOAc of in vitro callus cultures Astragalus vesicarius ssp. carniolicus, after enzymatic hydrolysis with β -glucosidase. Their structures tentatively elucidated by spectroscopic mean ( 1 H NMR HR-ESI-MS spectra). Antiproliferative activity extract aglycones against chemosensitive human promyelocyte cell line HL-60 its multidrug-resistant variant HL-60/Dox was assessed . Despite the strong (IC 50 8.8 µg/mL (HL-6, 72 h) to 11.8 (HL-60/Dox, h)), prenylated compound AV9 showed also antiproliferative 36.1 (HL-60 HL-60/Dox, h).
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ژورنال
عنوان ژورنال: ????????
سال: 2021
ISSN: ['2411-6076', '2709-135X']
DOI: https://doi.org/10.3897/pharmacia.68.e63421