Antioxidant, Antidiabetic and Structural Analysis of Spinacia Oleracea Leaf

Medicinal plants have been conventionally used to sustain health and treat many diseases such as diabetes for years. Spinacia oleracea L., common name palak, belong the class Amaranths family Amaranthaceous. It is an anti-bacterial, antidiabetic, anticancer, anti-inflammatory, antioxidant, hepatoprotective agent. In this study, methanol, ethanol, n-hexane aqueous extracts of leaf were evaluate antioxidant contents, activity, antidiabetic activity (inhibition glycation, alpha amylase, glucosidase, acetylchlinesterase). Additionally, identification bioactive compounds functional groups was done by Fourier transform infrared spectroscopy (FTIR) high performance liquid chromatography (HPLC). Among all extracts, total phenol flavonoid contents 32.09 ± 0.99 g GAE/100 78.38 1.15 22.77 0.16 CE/100 54.56 0.87 g. While DPPH reducing range 45.14% -75.77%. Methanol extract most potent it extracted maximum contents. Glycation amylase inhibition percentages 15.31 34.28 19.83-36.32 among tested samples spinach. Whereas, 12.67-43.77% 17.3% 28.04% blocking glucosidase acetylcholinesterase activities observed. HPLC analysis identified various flavonoids phenolics acids quercetin, gallic acid, caffeic sinopic cinnamic acid. FTIR showed that S. contains several phenols, amines, alkaloids, alcohols fluoro in methanolic leaves extract. This research indicated has wide potential be further investigated future can prove a wonderful natural drug healthcare systems upcoming