Antimicrobial susceptibility testing of mycoplasmas by ATP bioluminescence
نویسندگان
چکیده
منابع مشابه
Atp Bioluminescence Assay for Rapid Antibacterial Susceptibility Testing
We studied adenosine triphosphate (ATP) synthesized by bacteria in tube dilution cultures to devise a rapid antibacterial susceptibility test. The ATP level was determined with a firefly (Photinus pyralis) luciferin/luciferase bioluminescence assay, which allows a direct quantifying of bacteria above 10,000 CFU/ml. At sub-MIC, total bacterial ATP levels were paralleled by increased viable bacte...
متن کاملRapid antimicrobial susceptibility determination of uropathogens in clinical urine specimens by use of ATP bioluminescence.
We describe the first direct testing of the antimicrobial susceptibilities of bacterial pathogens in human clinical fluid samples by the use of ATP bioluminescence. We developed an ATP bioluminescence assay that eliminates somatic sources of ATP to selectively quantify the bacterial load in clinical urine specimens with a sensitivity of <1,000 CFU per milliliter. There was a log-log relationshi...
متن کاملRapid antibiotic susceptibility tests on Enterobacteriaceae by ATP bioluminescence.
The susceptibility of 76 clinical isolates of Enterobacteriaceae to ampicillin, piperacillin and gentamicin was assessed by ATP bioluminescence in a 4-h test. For most organisms tested (Escherichia, Klebsiella, Enterobacter and Serratia), there was good correlation with traditional MIC values estimated on 18-h cultures. However strains of Proteus mirabilis showed false resistance to the beta-la...
متن کاملA two-hour antibiotic susceptibility test by ATP-bioluminescence.
The antibiotic susceptibility test (AST) in Clinical Microbiology laboratories is still time-consuming, and most procedures take 24h to yield results. In this study, a rapid antimicrobial susceptibility test using ATP-bioluminescence has been developed. The design of method was performed using five ATCC collection strains of known susceptibility. This procedure was then validated against standa...
متن کاملAntimicrobial Susceptibility Testing of Cefoperazone
Cefoperazone is a new cephalosporin with a very wide spectrum of activity, including activity against Pseudomonas aeruginosa. It has less activity on enterococci and Acinetobacter. Of the 459 selected bacterial strains tested in this study, only 1.5% (7 strains and 6 genera) had minimum inhibitory concentrations of >128 ,ug/ml. For a minimum inhibitory concentration breakpoint of <32 p.g/ml (su...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Medical Microbiology
سال: 1991
ISSN: 0022-2615,1473-5644
DOI: 10.1099/00222615-35-2-89