Antimicrobial Activity of Identified Ubiquitin-40S Ribosomal Protein S27a (RPS27A), Ubiquitin-like Protein Fubi, and Ribosomal Protein (S30FAU) in the Starry Flounder (Platichthys stellatus)
نویسندگان
چکیده
Ubiquitin-40S ribosomal protein S27a (RPS27A), ubiquitin-like Fubi, and (S30FAU) are ubiquitin-related proteins that involved in the regulation of immune-related functions such as cell cycle, expression, apoptosis. This study aimed to confirm molecular characteristics, gene expression analysis, antibacterial activity RPS27A S30FAU identified from starry flounder (15 flounders 128.7 ± 18.2 g). An analysis using a normal fish showed was highly expressed head kidney, heart, stomach. In contrast, exhibited high stomach, kidney. Upon simulating an artificial pathogen infection, heart at 1 h 3 days post-viral hemorrhagic septicemia (VHSV) had liver, 7 post-Streptococcus parauberis (S. parauberis) infection. spleen gills day 12 post-VHSV respectively, kidney post-S. MIC antimicrobial against all bacteria used this study. biofilm assay, removed S. concentration-dependent manner, cytotoxicity test no hemolytic both S30FAU. Therefore, were confirmed possess peptide abilities without limitations cytotoxicity. provides valuable information on ability biology ubiquitin family isolated flounder.
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ژورنال
عنوان ژورنال: Fishes
سال: 2023
ISSN: ['2410-3888']
DOI: https://doi.org/10.3390/fishes8040187