Antiheparin proteins secreted by human platelets. purification, characterization, and radioimmunoassay
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چکیده
منابع مشابه
Antiheparin proteins secreted by human platelets. purification, characterization, and radioimmunoassay.
Platelet factor 4 (PF4) and low-affinity PF4 (LA-PF4) were purified to homogeneity from washed human platelets by ZnSO4 precipitation and affinity chromatography on heparinagarose columns. As shown by double immunodiffusion and specific radioimmunoassays, LA-PF4 and PF4 were immunologically distinct. The levels of PF4 and LA-PF4 antigens in intact platelets were 12.4 and 24.2 g/ i0 platelets, r...
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Two heparin-neutralizing proteins secreted by thrombin-stimulated platelets were purified to homogeneity by means of heparin-agarose affinity chromatography. These proteins, termed porcine platelet basic protein (PBP) and porcine platelet factor 4 (PF4), were eluted from a heparin-agarose column at 0.6-0.9 M NaCl and at 1-1.4 M NaCl, respectively. The molecular weight of porcine platelet basic ...
متن کاملHuman platelet basic protein associated with antiheparin and mitogenic activities: purification and partial characterization.
Platelet basic protein (PBP) (isoelectric point, 10.0-10.5; apparent Mr, 11,000-15,000) has been purified to homogeneity from material secreted by fresh human platelets after stimulation by thrombin. The purification, using preparative isoelectric focusing and chromatography on heparin-Sepharose, yielded two additional peptides with antiheparin activity that were immunologically identical with ...
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Three isozymes of diacylglycerol kinase (DGK), DGK-I, DGK-II, and DGK-III, were purified from the cytosol of human platelets by successive chromatography on DEAE-cellulose, Ultrogel AcA34, heparin-Sepharose, ATP-agarose, Mono Q, phenyl-Superose, HCA-hydroxyapatite, Wakopak G40, and TSK-3000SW columns. Two DGK species (DGK-I and DGK-III) were purified to apparent homogeneity, and upon SDS-polyac...
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Human and rhesus monkey platelets secrete at least two antiheparin proteins: platelet factor 4 (PF4) and low affinity platelet factor 4 (LA-PF4). Neither of these proteins showed species-related antigenic differences. As determined by radioimmunoassay, the levels of PF4 and LA-PF4 antigen per 10(9) monkey platelets amounted to 10.7 and 20.3 microgram, respectively. One milliliter of monkey plas...
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ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v53.1.47.47