Antigenicity of Japanese Encephalitis Virus Passage Serially in Chick Embryo Cell Culture

Association of virus specific replicative ribonucleic acid with nuclear membrane in chick embryo cells infected with japanese encephalitis virus.

Using high resolution electron microscopic autoradiography and velocity sedimentation, RNA synthesis was examined in chick embryo cells infected with Japanese encephalitis virus (JEV). RNA was labelled with 3H-uridine for 5 min or 10 min at 15 h after infection in the presence of actinomycin D and D-glucosamine. Microautoradiography showed significantly numbers of silver grains on the nuclear m...

Plaque formation of LaSota pathogenic strain of Newcastle disease virus adapted in chick embryo fibroblast cells

In order to adapt LaSota strain of Newcastle disease virus (NDV) on chick embryo fibroblast (CEF) cells, 0.1 ml from LaSota vaccine produced in Razi vaccine and serum research institute inoculated to the CEF cells and was passaged five times in the CEF grown in Eagle’s minimum essential medium (MEM). First to third passages were blind but in fourth passage cytopathic effect of virus was observe...

Evaluation and Optimization of Chick Embryo Fibroblasts for Production of a Fowl Pox Vaccine Based on Cell Culture

Abstract Background and Aims: For more than half a century, the production of fowl pox vaccine at Razi Vaccine and Serum Research Institute, has been carried out by injection method in the chick chorioallantoic membrane (CAM) and the vaccine has a favorable and effective in poultry flocks and has provided a complete satisfaction to the poultry flocks owner. Fowl pox vaccine is also manufactured...

Neuropathogenesis of Japanese encephalitis virus.

In the central nervous system, the Japanese encephalitis virus can replicate only in neurons. The mechanism of the type of neurotropism was analyzed. The susceptibility to Japanese encephalitis virus infection in the rat brain was closely associated with neuronal immaturity. The initial specific binding of the virus to cells is one of the reasons for neurotropism of the Japanese encephalitis vi...

Immunobiology of Japanese Encephalitis Virus