منابع مشابه
Antigen retrieval immunohistochemistry: past, present, and future.
The antigen retrieval (AR) technique, which is predominantly based on high-temperature heating of tissues, is used as a non-enzymatic pretreatment for immunohistochemical staining of formalin-fixed, paraffin-embedded tissue sections. It has been widely applied in pathology and analytical morphology. The existence of a growing body of literature on the AR technique raises a number of interesting...
متن کاملDenovirus Antigen Detection in Paraffinized Lung Sections of Pneumonic Goat Lungs Using Immunohistochemistry
BACKGROUNDS: Diseases affecting the respiratory tract of sheep and goats are one of the most important factors which limit production of these species on a world-wide basis. OBJECTIVES: The main goal of this study was to determine Adenovirus (AdV) antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic goats, using immunohistochemistry (IHC) staining method. METHODS: For this purpo...
متن کاملAntigen Retrieval Protocols
Antigen Retrieval Overview Overview of Heat-induced Epitope Retrieval Techniques and Devices Room Temperature Epitope Retrieval (RTER) Hydrochloric Acid Method (pH 1) Formic Acid Method (pH 2) Heat Induced Epitope Retrieval (HIER) Citrate Buffer Method (pH 6) Citrate-EDTA Buffer Method (pH 6.2) EDTA Method (pH 8) Tris-EDTA Method (pH 9) TBS Method (pH 9) Tris Buffer Method (pH...
متن کاملBHV-1 Antigen Detection in Paraffinized Lung Sections of Pneumonic Sheep Lung Using Immunohistochemistry
Background: Respiratory tract infections caused by some viruses with cattle origin have been demonstrated in sheep and goats. OBJECTIVES: The main goal of this study was to determine Bovine Herpes virus type 1 BHV1antigen in formalin-fixed paraffin-embedded lung tissue of pneumonic sheep, using immunohistochemistry (IHC) staining method. METHODS: For this purpose, the lungs of 4079 sheep, which...
متن کاملDistribution of Glucagon-Like Peptide (GLP)-2-Immunoreactive Cells in the Chicken Small Intestine: Antigen Retrieval Immunohistochemistry
An antigen retrieval method for immunohistochemical staining of glucagon-like peptide (GLP)-2-immunoreactive cells was investigated in the chicken small intestine. GLP-2-immunoreactive cells were observed as open-typed endocrine cells in the villous epithelium and crypts on both antigen retrieval agent-treated and untreated preparations. No obvious differences were detected in morphological fea...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 2011
ISSN: 0022-1554,1551-5044
DOI: 10.1369/jhc.2010.957191