Antigen Retrieval by Heating En Bloc for Pre-fixed Frozen Material

Role of Microwave Heating in Antigen Retrieval in Cryosections of Formalin-Fixed Tissues

Application of a Novel Venous Cannula for En-Bloc Removal of Undesirable Intravascular Material

Venous occlusive disease encompasses a variety of clinical entities that range the spectrum from being catastrophic and life threatening, such as massive pulmonary embolism, to disease states that may have an occult presentation, such as inferior vena cava occlusion. Other examples of veno-occlusive disease states include deep venous thrombosis and right atrial masses. When venous occlusion is ...

Arterial en bloc resection for pancreatic carcinoma.

BACKGROUND Surgery for locally advanced pancreatic cancer with arterial involvement of the hepatic artery, coeliac trunk and superior mesenteric artery (SMA) is highly controversial. In a retrospective review, the benefits and harms of arterial en bloc resection (AEBR) for pancreatic adenocarcinoma with arterial involvement were analysed. METHODS Patients were divided into three groups: 29 pa...

Antigen Retrieval Protocols

Antigen Retrieval Overview  Overview of Heat-induced Epitope Retrieval Techniques and Devices Room Temperature Epitope Retrieval (RTER)  Hydrochloric Acid Method (pH 1)  Formic Acid Method (pH 2) Heat Induced Epitope Retrieval (HIER)  Citrate Buffer Method (pH 6)  Citrate-EDTA Buffer Method (pH 6.2)  EDTA Method (pH 8)  Tris-EDTA Method (pH 9)  TBS Method (pH 9)  Tris Buffer Method (pH...

Novel reconstruction technique using a frozen tumor-bearing vertebra from a total en bloc sponydylectomy for spinal tumors.

This article describes a novel reconstruction technique using frozen tumor-bearing vertebrae in total en bloc spondylectomy for spinal tumors. Instead of harvesting autograft from the ilium or fibula, the resected lamina and vertebral body from the total en bloc spondylectomy are frozen in liquid nitrogen and used as grafted bone for spinal reconstruction. A retrospective review of 56 patients ...