Anticarcinogenic properties of malic acid on glioblastoma cell line through necrotic cell death mechanism

This study aimed to investigate the anticarcinogenic and genotoxic damage potentials of malic acid on human fibroblast cells (HDFa) glioblastoma (U87-MG) cell lines. MTT viability LDH release assays were performed understand cytotoxic features different Also, Hoechst 33258 fluorescent staining was used monitor nuclear abnormalities including micronucleus, lobbed, notched structures. Furthermore, cellular death mechanisms behind application investigated via use flow cytometry analysis. According analysis, showed a greater effect U87-MG compared HDFa line in terms cytotoxicity. Similarly, chromosomal integrity assay put forth higher number when lines, aberrations analyzed amplify concentration increased. Finally, analysis demonstrated necrotic than line. On other hand, apoptotic main mechanism against exposure In light these results, it can be concluded that concentrations, has an pathway, also shows properties When mutagenic are compared, could understood had impact cells.