Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

Immunohistochemistry is a widely used technique for research and diagnostic purposes that relies on the recognition by antibodies of antigens expressed in tissues. However, tissue processing particularly formalin fixation affect conformation these through formation methylene bridges. Although antigen retrieval techniques can partially restore immunoreactivity, it difficult to identify recognize their target especially formalin-fixed paraffin-embedded Most currently immunohistochemistry have been obtained animal immunization; however, vitro display represent alternative strategies not fully explored yet. This review provides an overview phage display-based antibody selections using naïve libraries various supports (fixed cells, dissociated tissues, fragments, sections) led identification suitable immunohistochemistry.