Anti-proliferative Effects of Hydroxytyrosol Against Breast Cancer Cell Lines Through Induction of Apoptosis

Background: Due to high mortality of patients with metastatic breast cancer and limited strategies for management this malignancy, development novel therapeutic approaches anti-cancer agents is essential. Objectives: In the present study, we investigated in vitro anti-proliferative effects hydroxytyrosol, as a natural chemotherapeutic agent, against cells MDA-MB-231 MCF-7. Methods: The anti-proliferation activity hydroxytyrosol on both MCF-7 was evaluated by MTT assay. Apoptosis percentage assessed flow cytometry treated untreated cells. Moreover, expression three apoptotic genes (BAX, BCL2, CASP3) real-time PCR Results: Our results indicated that exerted an appropriate dose- time-dependent manner. We observed significant increase apoptosis compared controls. addition, upregulated pro-apoptotic BAX CASP3 while downregulated anti-apoptotic BCL2 gene. Conclusions: findings study suggested effect may be due induction through modification