ANTI-INFLAMMATORY ACTIVITY OF ETHANOL EXTRACT AND ETHYL ACETATE FRACTION OF KEBIUL (Caesalpinia bonduc L.) SEED COAT AGAINST INHIBITION OF PROTEIN DENATURATION

Inflammation is a normal protective reaction against tissue damage caused by physical injury, harmful chemicals, and protein denaturation. Protein denaturation process in which proteins lose their tertiary structure secondary due to foreign substances, external compounds, such as strong acids, bases, organic salts, solvents, heating. The purpose of this study was determine the anti-inflammatory activity ethanol extract ethyl acetate fraction seed coat Kebiul (Caesalpinia bonduc L.) calculating IC50 value sample. results phytochemical test seeds contained flavonoids, alkaloids, terpenoids, steroids, saponins, tannins, while only tannins alkaloids. showed that highest percent inhibition sodium diclofenac, extract, fraction, respectively, at concentration 20 ppm 84.8%; 84.1%; 50%. values ​​of were 5.4 μg/mL; 9.9 13.3 μg/mL, respectively. three samples had exceeding 20% indicated all can be used an anti-inflammatory. Keywords: anti-inflammatory, denaturation, Caesalpinia L.