Anti-actin antibodies. An immunological approach to the myosin-actin and the tropomyosin-actin interfaces
نویسندگان
چکیده
منابع مشابه
Structure of the Rigor Actin-Tropomyosin-Myosin Complex
Regulation of myosin and filamentous actin interaction by tropomyosin is a central feature of contractile events in muscle and nonmuscle cells. However, little is known about molecular interactions within the complex and the trajectory of tropomyosin movement between its "open" and "closed" positions on the actin filament. Here, we report the 8 Å resolution structure of the rigor (nucleotide-fr...
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Tropomyosin is a regulatory protein associated with F-actin in many actomyosin contractile systems. If in vitro conditions are such that tropomyosin binds only slightly to F-actin, then the addition of myosin heads can induce stoichiometric binding between them. This suggests that formation of rigor bonds between actin and myosin heads may cause some change in the actin, stabilizing the appropr...
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The Problem: A long standing goal in engineering is to exploit the unique designs of biological systems to guide the development of autonomous biomimetic machines that exhibit agility, strength and speed in a variety of natural environments. Most critical to this effort is the development of an actuator that behaves like muscle. Although important research has been conducted to advance actuator...
متن کاملStudies on cardiac myofibrillogenesis with antibodies to titin, actin, tropomyosin, and myosin
Cardiac myofibrillogenesis was examined in cultured chick cardiac cells by immunofluorescence using antibodies against titin, actin, tropomyosin, and myosin. Primitive cardiomyocytes initially contained stress fiber-like structures (SFLS) that stained positively for alpha actin and/or muscle tropomyosin. In some cases the staining for muscle tropomyosin and alpha actin was disproportionate; thi...
متن کاملThe actin-myosin interface.
In order to understand the mechanism of muscle contraction at the atomic level, it is necessary to understand how myosin binds to actin in a reversible way. We have used a novel molecular dynamics technique constrained by an EM map of the actin-myosin complex at 13-A resolution to obtain an atomic model of the strong-binding (rigor) actin-myosin interface. The constraining force resulting from ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1987
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2440571